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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA009900LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA009900LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA009900LA01HU at dilution of 1:100

GRIA3 Antibody

CSB-PA009900LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGRIA3
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Overview

  • Supplier
    Cusabio
  • Product Name
    GRIA3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2892
  • Target name
    GRIA3
  • Target description
    glutamate ionotropic receptor AMPA type subunit 3
  • Target synonyms
    AMPA-selective glutamate receptor 3; dJ1171F9.1; GluA3; GLUR3; gluR-3; GLURC; GLUR-C; GLUR-K3; glutamate receptor 3; glutamate receptor C; glutamate receptor subunit 3; glutamate receptor, ionotrophic, AMPA 3; glutamate receptor, ionotropic, AMPA 3; MRX94; MRXSW
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42263
  • Protein Name
    Glutamate receptor 3
  • Scientific Description
    Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRIA3 using anti-GRIA3 antibody (PB9206). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA3 antigen affinity purified polyclonal antibody (Catalog # PB9206) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIA3 at approximately 101 kDa. The expected band size for GRIA3 is at 101 kDa.
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