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GLUT-1 (Tumor Progression and Mesothelioma Marker)(rGLUT1/2476), CF594 conjugate, 0.1mg/mL [26628-22-8]

GLUT-1 (Tumor Progression and Mesothelioma Marker)(rGLUT1/2476), CF594 conjugate, 0.1mg/mL [26628-22-8]

BNC943795
Biotium
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSLC2A1
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Overview

  • Supplier
    Biotium
  • Product Name
    GLUT-1 (Tumor Progression and Mesothelioma Marker)(rGLUT1/2476), CF594 conjugate, 0.1mg/mL
  • Delivery Days Customer
    9
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    rGLUT1/2476
  • Concentration
    0.1 mg/ml
  • Conjugate
    Other Conjugate
  • Gene ID6513
  • Target name
    SLC2A1
  • Target description
    solute carrier family 2 member 1
  • Target synonyms
    choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity); CSE; DYT17; DYT18; DYT9; EIG12; glucose transporter type 1, erythrocyte/brain; GLUT; GLUT1; GLUT-1; GLUT1DS; hepG2 glucose transporter; HTLVR; human T-cell leukemia virus (I and II) receptor; PED; receptor for HTLV-1 and HTLV-2; SDCHCN; solute carrier family 2 (facilitated glucose transporter), member 1; solute carrier family 2, facilitated glucose transporter member 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP11166
  • Protein Name
    Solute carrier family 2, facilitated glucose transporter member 1
  • Scientific Description
    Recognizes a protein of 55 kDa, which is identified as GLUT-1. Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are many types of glucose transport carrier proteins, designated as Glut-1 to Glut-12. Glut-1 is a major glucose transporter in the mammalian blood-brain barrier. It is expressed in high density on the membranes of human erythrocytes and the brain capillaries that comprise the blood-brain barrier. Glut-1 is expressed at variable levels in many human tissues. Overexpression of Glut-1 has been linked to tumor progression or poor survival of patients with carcinomas of the colon, breast, cervical, lung, bladder and mesothelioma. Glut-1 is a sensitive and specific marker for the differentiation of malignant mesothelioma (positive) from reactive mesothelium (negative). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
  • Source
    Animal
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC2A1 using anti-SLC2A1 antibody (PB9435). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate, Lane 2: mouse brain tissue lysate, Lane 3: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A1 antigen affinity purified polyclonal antibody (Catalog # PB9435) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A1 at approximately 55KD. The expected band size for SLC2A1 is at 55KD.
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