IHC-P analysis of human ovary adenocarcinoma tissue using GTX83628 GLUT5 antibody [9F3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
![IHC-P analysis of human endometrium tissue using GTX83628 GLUT5 antibody [9F3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83628/GTX83628_1630_IHC-P_w_23061420_600.webp "IHC-P analysis of human endometrium tissue using GTX83628 GLUT5 antibody [9F3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human ovary tissue using GTX83628 GLUT5 antibody [9F3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83628/GTX83628_1631_IHC-P_w_23061420_961.webp "IHC-P analysis of human ovary tissue using GTX83628 GLUT5 antibody [9F3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IP analysis of DDDDK tagged GLUT5 overexpressed HEK293T lysate using GTX83628 GLUT5 antibody [9F3].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX83628/GTX83628_3358_IP_w_23061420_157.webp "IP analysis of DDDDK tagged GLUT5 overexpressed HEK293T lysate using GTX83628 GLUT5 antibody [9F3].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate")
![WB analysis of HEK293T cells transfected with GLUT5 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83628 GLUT5 antibody [9F3]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83628/GTX83628_3777_WB_w_23061420_773.webp "WB analysis of HEK293T cells transfected with GLUT5 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83628 GLUT5 antibody [9F3]. Loading : 5 ug per lane")
![WB analysis of various cell lines using GTX83628 GLUT5 antibody [9F3]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83628/GTX83628_3778_WB_w_23061420_581.webp "WB analysis of various cell lines using GTX83628 GLUT5 antibody [9F3]. Loading : 35 ug per lane")
![FACS analysis of HeLa cells using GTX83628 GLUT5 antibody [9F3]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX83628/GTX83628_98_FACS_w_23061420_190.webp "FACS analysis of HeLa cells using GTX83628 GLUT5 antibody [9F3]. Red : Primary antibody Blue : Negative control antibody")
![FACS analysis of Jurkat cells using GTX83628 GLUT5 antibody [9F3]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX83628/GTX83628_99_FACS_w_23061420_933.webp "FACS analysis of Jurkat cells using GTX83628 GLUT5 antibody [9F3]. Red : Primary antibody Blue : Negative control antibody")
IHC-P analysis of human ovary adenocarcinoma tissue using GTX83628 GLUT5 antibody [9F3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
GLUT5 antibody [9F3]
GTX83628
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetSLC2A5
Overview
- SupplierGeneTex
- Product NameGLUT5 antibody [9F3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:200-1:1000. IHC-P: 1:50. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID9F3
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6518
- Target nameSLC2A5
- Target descriptionsolute carrier family 2 member 5
- Target synonymsGLUT-5, GLUT5, solute carrier family 2, facilitated glucose transporter member 5, glucose transporter type 5, small intestine, glucose transporter-like protein 5, solute carrier family 2 (facilitated glucose/fructose transporter), member 5, testicular tissue protein Li 81
- HostMouse
- IsotypeIgG1
- Protein IDP22732
- Protein NameSolute carrier family 2, facilitated glucose transporter member 5
- Scientific DescriptionCytochalasin B-sensitive carrier. Seems to function primarily as a fructose transporter.
- ReactivityHuman, Monkey, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Evangelista-Silva PH, Prates RP, Leite JSM, et al. Intestinal GLUT5 and FAT/CD36 transporters and blood glucose are reduced by a carotenoid/MUFA-rich oil in high-fat fed mice. Life Sci. 2021,279:119672. doi: 10.1016/j.lfs.2021.119672Read this paper
- Torelli Hijo AH, Coutinho CP, Alba-Loureiro TC, et al. High fat diet modulates the protein content of nutrient transporters in the small intestine of mice: possible involvement of PKA and PKC activity. Heliyon. 2019,5(10):e02611. doi: 10.1016/j.heliyon.2019.e02611Read this paper
- Losacco MC, de Almeida CFT, Hijo AHT, et al. High-fat diet affects gut nutrients transporters in hypo and hyperthyroid mice by PPAR-a independent mechanism. Life Sci. 2018,202:35-43. doi: 10.1016/j.lfs.2018.03.053Read this paper
Datasheet
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