![IHC-P analysis of human endometrium tissue using GTX84426 Glutamine synthetase antibody [1F4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human endometrium tissue using GTX84426 Glutamine synthetase antibody [1F4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84426/GTX84426_2705_IHC-P_w_23061420_245.webp)
IHC-P analysis of human endometrium tissue using GTX84426 Glutamine synthetase antibody [1F4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
Glutamine synthetase antibody [1F4]
GTX84426
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetGLUL
Overview
- SupplierGeneTex
- Product NameGlutamine synthetase antibody [1F4]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. ICC/IF: 1:50-1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1F4
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2752
- Target nameGLUL
- Target descriptionglutamate-ammonia ligase
- Target synonymsDEE116, GLNS, GS, PIG43, PIG59, glutamine synthetase, cell proliferation-inducing protein 59, glutamate decarboxylase, glutamine synthase, palmitoyltransferase GLUL, proliferation-inducing protein 43
- HostMouse
- IsotypeIgG2a
- Protein IDP15104
- Protein NameGlutamine synthetase
- Scientific DescriptionThis enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Guenther SR, Schumann RG, Zaytseva Y, et al. Cell composition at the vitreomacular interface in traumatic macular holes. Graefes Arch Clin Exp Ophthalmol. 2022,260(3):873-884. doi: 10.1007/s00417-021-05470-zRead this paper
![FACS analysis of Jurkat cells using GTX84426 Glutamine synthetase antibody [1F4]. Red : Primary antibody Blue : Negative control antibody FACS analysis of Jurkat cells using GTX84426 Glutamine synthetase antibody [1F4]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX84426/GTX84426_416_FACS_w_23061420_593.webp)
![WB analysis of HEK293T cells transfected with Glutamine synthetase plasmid (Right) or empty vector (Left) for 48 hrs using GTX84426 Glutamine synthetase antibody [1F4]. Loading : 5 ug per lane WB analysis of HEK293T cells transfected with Glutamine synthetase plasmid (Right) or empty vector (Left) for 48 hrs using GTX84426 Glutamine synthetase antibody [1F4]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84426/GTX84426_4365_WB_w_23061420_436.webp)
![IHC-P analysis of human breast tissue using GTX84426 Glutamine synthetase antibody [1F4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human breast tissue using GTX84426 Glutamine synthetase antibody [1F4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84426/GTX84426_2704_IHC-P_w_23061420_163.webp)
![FACS analysis of HeLa cells using GTX84426 Glutamine synthetase antibody [1F4]. Red : Primary antibody Blue : Negative control antibody FACS analysis of HeLa cells using GTX84426 Glutamine synthetase antibody [1F4]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX84426/GTX84426_415_FACS_w_23061420_762.webp)
![ICC/IF analysis of HepG2 cells using GTX84426 Glutamine synthetase antibody [1F4]. ICC/IF analysis of HepG2 cells using GTX84426 Glutamine synthetase antibody [1F4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84426/GTX84426_1080_ICCIF_w_23061420_764.webp)
![WB analysis of various cell lines using GTX84426 Glutamine synthetase antibody [1F4]. Loading : 35 ug per lane WB analysis of various cell lines using GTX84426 Glutamine synthetase antibody [1F4]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84426/GTX84426_4366_WB_w_23061420_570.webp)
![IHC-P analysis of human ovary tissue using GTX84426 Glutamine synthetase antibody [1F4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. IHC-P analysis of human ovary tissue using GTX84426 Glutamine synthetase antibody [1F4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84426/GTX84426_2706_IHC-P_w_23061420_565.webp)
![ICC/IF analysis of COS7 cells transiently transfected with Glutamine synthetase plasmid using GTX84426 Glutamine synthetase antibody [1F4]. ICC/IF analysis of COS7 cells transiently transfected with Glutamine synthetase plasmid using GTX84426 Glutamine synthetase antibody [1F4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84426/GTX84426_1079_ICCIF_w_23061420_952.webp)

![IHC-P analysis of human liver tissue using GTX04471 Glutamine synthetase antibody [MSVA-750M] HistoMAX?. Glutamine synthetase staining is strong in centrilobular hepatocytes weak to moderate in Kupffer cells but absent in periportal hepatocytes.](https://www.genetex.com/upload/website/prouct_img/normal/GTX04471/GTX04471_20230728_IHC-P_57_23072722_662.webp)

![Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GTX630654) diluted at 1:5000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630654/GTX630654_41603_20160901_WB_R_brain_w_23061202_653.webp)

![Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638336/GTX638336_T-44977_20230317_WB_M_R_23032022_129.webp)
