Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638336/GTX638336_T-44977_20230317_WB_shRNA_watermark_23032022_873.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638336/GTX638336_T-44977_20230407_IHC-P_multiple_M_23041719_834.webp "Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638336/GTX638336_T-44977_20230407_IHC-P_multiple_R_23041719_383.webp "Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638336/GTX638336_45047_20230519_WB_TPM_watermark_23053001_968.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638336/GTX638336_45047_20230616_WB_D_23062019_194.webp "Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638336/GTX638336_45047_20230707_WB_B_23071223_930.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat cerebellum. Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638336/GTX638336_45047_20230721_IHC-P_Cat_23073119_156.webp "Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat cerebellum. Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein at glia by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638336/GTX638336_T-44977_20231222_ICC_IF_R_24011618_545.webp "Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein at glia by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Zebrafish tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638336/GTX638336_45047_20241004_WB_Z_brain_24100900_594.webp "Zebrafish tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Glutamine synthetase antibody [HL2283]
GTX638336
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Feline, Human, Mouse, Rat, Zebra Fish
TargetGLUL
Overview
- SupplierGeneTex
- Product NameGlutamine synthetase antibody [HL2283]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2283
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2752
- Target nameGLUL
- Target descriptionglutamate-ammonia ligase
- Target synonymsDEE116, GLNS, GS, PIG43, PIG59, glutamine synthetase, cell proliferation-inducing protein 59, glutamate decarboxylase, glutamine synthase, palmitoyltransferase GLUL, proliferation-inducing protein 43
- HostRabbit
- IsotypeIgG
- Protein IDP15104
- Protein NameGlutamine synthetase
- Scientific DescriptionThe protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
- ReactivityCanine, Feline, Human, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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