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Glutathione Reductase antibody [N2C2], Internal

GTX114199
GeneTex
ApplicationsElectron Microscopy, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGSR
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Overview

  • Supplier
    GeneTex
  • Product Name
    Glutathione Reductase antibody [N2C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Electron Microscopy, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.21 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2936
  • Target name
    GSR
  • Target description
    glutathione-disulfide reductase
  • Target synonyms
    epididymis luminal protein 75; epididymis secretory sperm binding protein Li 122m; glutathione reductase, mitochondrial; glutathione S-reductase; GR; GRase; GSRD; HEL-75; HEL-S-122m
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00390
  • Protein Name
    Glutathione reductase, mitochondrial
  • Scientific Description
    This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Downregulation of TAZ elicits a mitochondrial redox imbalance and ferroptosis in lung epithelial cells exposed to diesel exhaust particles.
    Read more
  • Beneficial Effects of Glucagon-Like Peptide-1 (GLP-1) in Diabetes-Induced Retinal Abnormalities: Involvement of Oxidative Stress. Ramos H et al., 2020 Sep 10, Antioxidants (Basel)
    Read more
  • The immunohistochemical and histologic effects of contrast medium on uterus, fallopian tubes and ovaries, given during hysterosalpingography: rat study. Pek E et al., 2020 Dec 4, J Turk Ger Gynecol Assoc
    Read more
  • Sesamin Enhances Nrf2-Mediated Protective Defense against Oxidative Stress and Inflammation in Colitis via AKT and ERK Activation. Bai X et al., 2019, Oxid Med Cell Longev
    Read more
  • Choroid plexus glutathione peroxidases are instrumental in protecting the brain fluid environment from hydroperoxides during postnatal development. Saudrais E et al., 2018 Oct 1, Am J Physiol Cell Physiol
    Read more
  • Taurine treatment prevents derangement of the hepatic gamma-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism. Maclean KN et al., 2018 Mar, FASEB J
    Read more
  • Angiotensin II type 1a receptor-deficient mice develop angiotensin II-induced oxidative stress and DNA damage without blood pressure increase. Zimnol A et al., 2017 Dec 1, Am J Physiol Renal Physiol
    Read more
  • Synthetic beta-nitrostyrene derivative CYT-Rx20 as inhibitor of oral cancer cell proliferation and tumor growth through glutathione suppression and reactive oxygen species induction. Wang YY et al., 2017 Jun, Head Neck
    Read more
  • Shikonin inhibits oxidized LDL-induced monocyte adhesion by suppressing NFkappaB activation via up-regulation of PI3K/Akt/Nrf2-dependent antioxidation in EA.hy926 endothelial cells. Huang CS et al., 2015 Feb 1, Biochem Pharmacol
    Read more
  • Effect of glutathione reductase knockdown in response to UVB-induced oxidative stress in human lung adenocarcinoma. Chou HC et al., 2014 Jan 9, Proteome Sci
    Read more

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Figure 1. Western blot analysis of Glutathione Reductase using anti-Glutathione Reductase antibody (A01479-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse kidney tissue lysates, Lane 9: mouse colon tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glutathione Reductase antigen affinity purified polyclonal antibody (Catalog # A01479-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glutathione Reductase at approximately 52 kDa. The expected band size for Glutathione Reductase is at 56 kDa.
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