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Glutathione Reductase Antibody (YA403, Rabbit)

HY-P80693
MedChem Express
TargetGSR
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Glutathione Reductase Antibody (YA403, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2936
  • Target name
    GSR
  • Target description
    glutathione-disulfide reductase
  • Target synonyms
    CNSHA10, GR, GSRD, HEL-75, HEL-S-122m, glutathione reductase, mitochondrial, GRase, epididymis luminal protein 75, epididymis secretory sperm binding protein Li 122m, glutathione S-reductase
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Glutathione Reductase Antibody (YA403) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glutathione Reductase.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Glutathione Reductase using anti-Glutathione Reductase antibody (A01479-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: human Daudi whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse kidney tissue lysates, Lane 9: mouse colon tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glutathione Reductase antigen affinity purified polyclonal antibody (Catalog # A01479-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glutathione Reductase at approximately 52 kDa. The expected band size for Glutathione Reductase is at 56 kDa.
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