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Glycine Dehydrogenase (GLDC) Polyclonal Antibody

CAU22233
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetGLDC
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Overview

  • Supplier
    Biomatik
  • Product Name
    Glycine Dehydrogenase (GLDC) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2731
  • Target name
    GLDC
  • Target description
    glycine decarboxylase
  • Target synonyms
    GCE; GCSP; glycine cleavage system protein P; glycine decarboxylase P-protein; glycine dehydrogenase (aminomethyl-transferring); glycine dehydrogenase (decarboxylating), mitochondrial; glycine dehydrogenase [decarboxylating], mitochondrial; HYGN1; nonketotic hyperglycinemia
  • Host
    Rabbit
  • Protein IDP23378
  • Protein Name
    Glycine dehydrogenase (decarboxylating), mitochondrial
  • Scientific Description
    The Glycine Dehydrogenase (GLDC) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Glycine decarboxylase/GLDC using anti-Glycine decarboxylase/GLDC antibody (A04777-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEPG2 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human T47D whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse liver tissue lysates, Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glycine decarboxylase/GLDC antigen affinity purified polyclonal antibody (Catalog # A04777-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glycine decarboxylase/GLDC at approximately 113KD. The expected band size for Glycine decarboxylase/GLDC is at 113KD.
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