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Staining is restricted to the glandular cells in formalin-fixed paraffin-embedded endometrial tissue from the late secretory phase, stained with GTX60956
Staining is restricted to the glandular cells in formalin-fixed paraffin-embedded endometrial tissue from the late secretory phase, stained with GTX60956
Staining is restricted to the glandular cells in formalin-fixed paraffin-embedded endometrial tissue from the late secretory phase, stained with GTX60956

Glycodelin antibody [001-13]

GTX60956
GeneTex
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPAEP
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Overview

  • Supplier
    GeneTex
  • Product Name
    Glycodelin antibody [001-13]
  • Delivery Days Customer
    9
  • Applications
    ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    001-13
  • Conjugate
    Unconjugated
  • Gene ID5047
  • Target name
    PAEP
  • Target description
    progestagen associated endometrial protein
  • Target synonyms
    alpha uterine protein; GD; GdA; GdF; GdS; glycodelin; glycodelin-A; glycodelin-F; glycodelin-S; PAEG; PEG; PEP; placental protein 14; PP14; PP14 protein (placental protein 14); pregnancy-associated endometrial alpha-2 globulin; progestagen-associated endometrial protein (placental protein 14, pregnancy-associated endometrial a; progesterone-associated endometrial protein; ZIF-1; zona-binding inhibitory factor-1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP09466
  • Protein Name
    Glycodelin
  • Scientific Description
    This gene is a member of the kernel lipocalin superfamily whose members share relatively low sequence similarity but have highly conserved exon/intron structure and three-dimensional protein folding. Most lipocalins are clustered on the long arm of chromosome 9. The encoded glycoprotein has been previously referred to as pregnancy-associated endometrial alpha-2-globulin, placental protein 14, and glycodelin, but has been officially named progestagen-associated endometrial protein. Three distinct forms, with identical protein backbones but different glycosylation profiles, are found in amniotic fluid, follicular fluid and seminal plasma of the reproductive system. These glycoproteins have distinct and essential roles in regulating a uterine environment suitable for pregnancy and in the timing and occurrence of the appropriate sequence of events in the fertilization process. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Glycodelin/PAEP using anti-Glycodelin/PAEP antibody (A06051-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glycodelin/PAEP antigen affinity purified polyclonal antibody (Catalog # A06051-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glycodelin/PAEP at approximately 24 kDa. The expected band size for Glycodelin/PAEP is at 21,24-28 kDa.
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