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ProductsBack/PAEP / Glycodelin / GdF Antibody

PAEP / Glycodelin / GdF Antibody

LS-C401721
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPAEP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PAEP / Glycodelin / GdF Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:100 - 1:300), WB (1:200 - 1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.8 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5047
  • Target name
    PAEP
  • Target description
    progestagen associated endometrial protein
  • Target synonyms
    GD, GdA, GdF, GdS, PAEG, PEP, PP14, ZIF-1, glycodelin, PEG, PP14 protein (placental protein 14), alpha uterine protein, glycodelin-A, glycodelin-F, glycodelin-S, placental protein 14, pregnancy-associated endometrial alpha-2 globulin, progestagen-associated endometrial protein (placental protein 14, pregnancy-associated endometrial a, progesterone-associated endometrial protein, zona-binding inhibitory factor-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Glycodelin/PAEP using anti-Glycodelin/PAEP antibody (A06051-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glycodelin/PAEP antigen affinity purified polyclonal antibody (Catalog # A06051-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glycodelin/PAEP at approximately 24 kDa. The expected band size for Glycodelin/PAEP is at 21,24-28 kDa.
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