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PAEP Polyclonal Antibody

CAC14747
Biomatik
ApplicationsImmunoPrecipitation, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPAEP
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Overview

  • Supplier
    Biomatik
  • Product Name
    PAEP Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5047
  • Target name
    PAEP
  • Target description
    progestagen associated endometrial protein
  • Target synonyms
    alpha uterine protein; GD; GdA; GdF; GdS; glycodelin; glycodelin-A; glycodelin-F; glycodelin-S; PAEG; PEG; PEP; placental protein 14; PP14; PP14 protein (placental protein 14); pregnancy-associated endometrial alpha-2 globulin; progestagen-associated endometrial protein (placental protein 14, pregnancy-associated endometrial a; progesterone-associated endometrial protein; ZIF-1; zona-binding inhibitory factor-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09466
  • Protein Name
    Glycodelin
  • Scientific Description
    The PAEP Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, IHC, IP.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Glycodelin/PAEP using anti-Glycodelin/PAEP antibody (A06051-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glycodelin/PAEP antigen affinity purified polyclonal antibody (Catalog # A06051-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glycodelin/PAEP at approximately 24 kDa. The expected band size for Glycodelin/PAEP is at 21,24-28 kDa.
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