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ProductsBack/Glypican 3 (GPC3) Mouse Monoclonal Antibody [Clone ID: 9C2]
Western blot analysis using GPC3 antibody against HepG2 (Lane 1), HEK293 (Lane 2), Jurkat (Lane 3), SK-N-SH (Lane 4), PC-12 (Lane 5), F9 (Lane 6)and Mouse liver (Lane 7) cell lysate.
Western blot analysis using GPC3 antibody against HepG2 (Lane 1), HEK293 (Lane 2), Jurkat (Lane 3), SK-N-SH (Lane 4), PC-12 (Lane 5), F9 (Lane 6)and Mouse liver (Lane 7) cell lysate.
Western blot analysis using GPC3 antibody against HepG2 (Lane 1), HEK293 (Lane 2), Jurkat (Lane 3), SK-N-SH (Lane 4), PC-12 (Lane 5), F9 (Lane 6)and Mouse liver (Lane 7) cell lysate.

Glypican 3 (GPC3) Mouse Monoclonal Antibody [Clone ID: 9C2]

AM06776PU-N
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetGPC3
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Glypican 3 (GPC3) Mouse Monoclonal Antibody [Clone ID: 9C2]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    9C2
  • Gene ID2719
  • Target name
    GPC3
  • Target description
    glypican 3
  • Target synonyms
    DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1, glypican-3, glypican proteoglycan 3, heparan sulphate proteoglycan, intestinal protein OCI-5, secreted glypican-3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP51654
  • Protein Name
    Glypican-3
  • Scientific Description
    Glypican 3 (GPC3) mouse monoclonal antibody, clone 9C2, Purified
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Glypican 3/GPC3 using anti-Glypican 3/GPC3 antibody (A01922-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glypican 3/GPC3 antigen affinity purified polyclonal antibody (Catalog # A01922-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glypican 3/GPC3 at approximately 66 kDa. The expected band size for Glypican 3/GPC3 is at 66 kDa.
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