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Cross-reactivity assessment of Glypican-3 Mouse Recombinant Antibody AE00138 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
Cross-reactivity assessment of Glypican-3 Mouse Recombinant Antibody AE00138 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.
Cross-reactivity assessment of Glypican-3 Mouse Recombinant Antibody AE00138 (1ug/ml) on CDI’s Protein Array containing more than 19,000 full-length human proteins.

Glypican-3 Recombinant Antibody AE00138

AE00138
Aeonian Biotech
TargetGPC3
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Product group Antibodies
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Overview

  • Supplier
    Aeonian Biotech
  • Product Name
    Glypican-3 Recombinant Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    ICC, IHC, PA
  • Category Supplier
    Antibody
  • Certification
    Research Use Only
  • Clonality
    Recombinant
  • Clone ID
    rGPC3/863
  • Conjugate
    Unconjugated
  • Gene ID2719
  • Target name
    GPC3
  • Target description
    glypican 3
  • Target synonyms
    DGSX; glypican proteoglycan 3; glypican-3; GTR2-2; heparan sulphate proteoglycan; intestinal protein OCI-5; MXR7; OCI-5; SDYS; secreted glypican-3; SGB; SGBS; SGBS1
  • Host
    Mouse
  • Isotype
    IgG1 kappa
  • Protein IDP51654
  • Protein Name
    Glypican-3
  • Scientific Description
    Glypican-3 Recombinant Antibody AE00138
  • Shelf life instruction
    Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
  • Reactivity Supplier
    Human
  • Storage Instruction
    2-8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Glypican 3/GPC3 using anti-Glypican 3/GPC3 antibody (A01922-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glypican 3/GPC3 antigen affinity purified polyclonal antibody (Catalog # A01922-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glypican 3/GPC3 at approximately 66 kDa. The expected band size for Glypican 3/GPC3 is at 66 kDa.
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