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GMDS Antibody

CAB15060
Assay Genie
TargetTET1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    GMDS Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID80312
  • Target name
    TET1
  • Target description
    tet methylcytosine dioxygenase 1
  • Target synonyms
    CXXC6, LCX, bA119F7.1, methylcytosine dioxygenase TET1, CXXC finger 6, CXXC zinc finger 6, CXXC-type zinc finger protein 6, leukemia-associated protein with a CXXC domain, ten-eleven translocation 1 gene protein, ten-eleven translocation-1, tet oncogene 1
  • Host
    Rabbit
  • Protein IDQ8NFU7
  • Protein Name
    Methylcytosine dioxygenase TET1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TET1 using anti-TET1 antibody (A00603-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: monkey Cos-7 whole cell lysates, Lane 6: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TET1 antigen affinity purified polyclonal antibody (Catalog # A00603-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TET1 at approximately 235KD. The expected band size for TET1 is at 235KD.
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