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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA009577LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA009577LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA009577LA01HU at dilution of 1:100

GMNN Antibody

CSB-PA009577LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGMNN
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Overview

  • Supplier
    Cusabio
  • Product Name
    GMNN Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51053
  • Target name
    GMNN
  • Target description
    geminin DNA replication inhibitor
  • Target synonyms
    Gem; geminin; MGORS6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75496
  • Protein Name
    Geminin
  • Scientific Description
    Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Geminin/GMNN using anti-Geminin/GMNN antibody (A06060-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: human SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Geminin/GMNN antigen affinity purified polyclonal antibody (Catalog # A06060-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Geminin/GMNN at approximately 25 kDa. The expected band size for Geminin/GMNN is at 25 kDa.
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