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Western Blot analysis of HEPG2-UV cells using Galpha olf Polyclonal Antibody
Western Blot analysis of HEPG2-UV cells using Galpha olf Polyclonal Antibody
Western Blot analysis of HEPG2-UV cells using Galpha olf Polyclonal Antibody

GNAL Antibody

CSB-PA002857
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGNAL
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Overview

  • Supplier
    Cusabio
  • Product Name
    GNAL Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2774
  • Target name
    GNAL
  • Target description
    G protein subunit alpha L
  • Target synonyms
    adenylate cyclase-stimulating G alpha protein, olfactory type; DYT25; guanine nucleotide binding protein (G protein), alpha activating activity polypeptide, olfactory type; guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide, olfactory type; guanine nucleotide-binding protein G(olf) subunit alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP38405
  • Protein Name
    Guanine nucleotide-binding protein G(olf) subunit alpha
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GNAL using anti-GNAL antibody (A09404-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNAL antigen affinity purified polyclonal antibody (Catalog # A09404-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNAL at approximately 44 kDa. The expected band size for GNAL is at 44 kDa.
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