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Western Blot analysis of HEPG2-UV cells using Galpha olf Polyclonal Antibody
Western Blot analysis of HEPG2-UV cells using Galpha olf Polyclonal Antibody
Western Blot analysis of HEPG2-UV cells using Galpha olf Polyclonal Antibody

GNAL Antibody

CSB-PA002857
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGNAL
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Overview

  • Supplier
    Cusabio
  • Product Name
    GNAL Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2774
  • Target name
    GNAL
  • Target description
    G protein subunit alpha L
  • Target synonyms
    DYT25, HG1O, guanine nucleotide-binding protein G(olf) subunit alpha, adenylate cyclase-stimulating G alpha protein, olfactory type, guanine nucleotide binding protein (G protein), alpha activating activity polypeptide, olfactory type, guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide, olfactory type, heterotrimeric guanine nucleotide-binding protein 1O
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP38405
  • Protein Name
    Guanine nucleotide-binding protein G(olf) subunit alpha
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GNAL using anti-GNAL antibody (A09404-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNAL antigen affinity purified polyclonal antibody (Catalog # A09404-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNAL at approximately 44 kDa. The expected band size for GNAL is at 44 kDa.
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