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Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-5: Raji cells, 293T cells, LoVo cells, Hela cells, K562 cells, Primary antibody: CSB-PA184930(GNL3 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-5: Raji cells, 293T cells, LoVo cells, Hela cells, K562 cells, Primary antibody: CSB-PA184930(GNL3 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-5: Raji cells, 293T cells, LoVo cells, Hela cells, K562 cells, Primary antibody: CSB-PA184930(GNL3 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds

GNL3 Antibody

CSB-PA184930
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetGNL3
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Overview

  • Supplier
    Cusabio
  • Product Name
    GNL3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26354
  • Target name
    GNL3
  • Target description
    G protein nucleolar 3
  • Target synonyms
    C77032, E2IG3, NNP47, NS, Nug1, guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, estradiol-induced nucleotide binding protein, guanine nucleotide binding protein-like 3 (nucleolar), novel nucleolar protein 47, nucleolar GTP-binding protein 3, nucleostemin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BVP2
  • Protein Name
    Guanine nucleotide-binding protein-like 3
  • Scientific Description
    The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Nucleostemin/GNL3 using anti-Nucleostemin/GNL3 antibody (A03283-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nucleostemin/GNL3 antigen affinity purified polyclonal antibody (Catalog # A03283-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nucleostemin/GNL3 at approximately 65 kDa. The expected band size for Nucleostemin/GNL3 is at 65 kDa.
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