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ProductsBack/GNL3 / NS / Nucleostemin Antibody (aa1-226, clone 2C8D5)

GNL3 / NS / Nucleostemin Antibody (aa1-226, clone 2C8D5)

LS-C191783
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetGNL3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GNL3 / NS / Nucleostemin Antibody (aa1-226, clone 2C8D5)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), Flo (1:200 - 1:400), WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2C8D5
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID26354
  • Target name
    GNL3
  • Target description
    G protein nucleolar 3
  • Target synonyms
    C77032; E2IG3; E2-induced gene 3 protein; estradiol-induced nucleotide binding protein; guanine nucleotide binding protein-like 3 (nucleolar); guanine nucleotide-binding protein-like 3; NNP47; novel nucleolar protein 47; NS; nucleolar GTP-binding protein 3; nucleostemin
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nucleostemin/GNL3 using anti-Nucleostemin/GNL3 antibody (A03283-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nucleostemin/GNL3 antigen affinity purified polyclonal antibody (Catalog # A03283-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nucleostemin/GNL3 at approximately 65 kDa. The expected band size for Nucleostemin/GNL3 is at 65 kDa.
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