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Goat anti-ALDH18A1

EB10433
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetALDH18A1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ALDH18A1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5832
  • Target name
    ALDH18A1
  • Target description
    aldehyde dehydrogenase 18 family member A1
  • Target synonyms
    ADCL3, ARCL3A, GSAS, P5CS, PYCS, SPG9, SPG9A, SPG9B, delta-1-pyrroline-5-carboxylate synthase, Spastic paraplegia-9 (spastic paraparesis with amyotrophy, cataracts and gastroesophageal reflux), aldehyde dehydrogenase family 18 member A1, delta-1-pyrroline-5-carboxylate synthetase, delta1-pyrroline-5-carboxlate synthetase, pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase), spastic paraplegia 9 (autosomal dominant)
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002851.2; NP_001017423.1. GeneIDs all Nonhuman: 56454 (mouse); 361755 (rat);. Purification: Antigen affinity purified. Names and symbols: ALDH18A1; aldehyde dehydrogenase 18 family, member A1; GSAS; MGC117316; P5CS; PYCS; aldehyde dehydrogenase 18A1; delta1-pyrroline-5-carboxlate synthetase; pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase)
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow, Pig
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ALDH18A1 using anti-ALDH18A1 antibody (A06119-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH18A1 antigen affinity purified polyclonal antibody (Catalog # A06119-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH18A1 at approximately 87KD. The expected band size for ALDH18A1 is at 87KD.
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