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Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.

Anti-ALDH18A1 Antibody

HPA008333
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetALDH18A1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ALDH18A1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5832
  • Target name
    ALDH18A1
  • Target description
    aldehyde dehydrogenase 18 family member A1
  • Target synonyms
    ADCL3, ARCL3A, GSAS, P5CS, PYCS, SPG9, SPG9A, SPG9B, delta-1-pyrroline-5-carboxylate synthase, Spastic paraplegia-9 (spastic paraparesis with amyotrophy, cataracts and gastroesophageal reflux), aldehyde dehydrogenase family 18 member A1, delta-1-pyrroline-5-carboxylate synthetase, delta1-pyrroline-5-carboxlate synthetase, pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase), spastic paraplegia 9 (autosomal dominant)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54886
  • Protein Name
    Delta-1-pyrroline-5-carboxylate synthase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of ALDH18A1 using anti-ALDH18A1 antibody (A06119-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH18A1 antigen affinity purified polyclonal antibody (Catalog # A06119-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH18A1 at approximately 87KD. The expected band size for ALDH18A1 is at 87KD.
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