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ProductsBack/Goat anti-ALDH6A1 (aa487-496)

Goat anti-ALDH6A1 (aa487-496)

EB10437
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetALDH6A1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ALDH6A1 (aa487-496) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID4329
  • Target name
    ALDH6A1
  • Target description
    aldehyde dehydrogenase 6 family member A1
  • Target synonyms
    malonate-semialdehyde dehydrogenase (acetylating); methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial; mitochondrial acylating methylmalonate-semialdehyde dehydrogenase; MMSADHA; MMSDH; testicular tissue protein Li 122
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_005580.1; NP_001265522.1; NP_001265523.1. GeneIDs all Nonhuman: 104776 (mouse); 81708 (rat);. Purification: Antigen affinity purified. Names and symbols: ALDH6A1; aldehyde dehydrogenase 6 family, member A1; MGC40271; MMSADHA; MMSDH; aldehyde dehydrogenase 6A1; mitochondrial acylating methylmalonate-semialdehyde dehydrogenase
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow, Pig
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Mouse kidney tissue, Mouse liver tissue All lanes: ALDH6A1 antibody at 2.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58, 57 kDa Observed band size: 58 kDa
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Figure 1. Western blot analysis of ALDH6A1 using anti-ALDH6A1 antibody (A08868-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: human HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH6A1 antigen affinity purified polyclonal antibody (Catalog # A08868-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH6A1 at approximately 58KD. The expected band size for ALDH6A1 is at 58KD.
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