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ProductsBack/Goat anti-APOBEC3C (C-term)

Goat anti-APOBEC3C (C-term)

EB08307
Everest Biotech
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetAPOBEC3C
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-APOBEC3C (C-term) Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID27350
  • Target name
    APOBEC3C
  • Target description
    apolipoprotein B mRNA editing enzyme catalytic subunit 3C
  • Target synonyms
    A3C, APOBEC1L, ARDC2, ARDC4, ARP5, PBI, bK150C2.3, DNA dC->dU-editing enzyme APOBEC-3C, apolipoprotein B editing enzyme catalytic polypeptide-like 3C, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3C, phorbolin I, probable DNA dC->dU-editing enzyme APOBEC-3C
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_055323.2. Purification: Antigen affinity purified. Names and symbols: APOBEC3C ; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3C ; APOBEC1L; ARDC2; ARDC4; ARP5; MGC19485; PBI; bK150C2.3
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of APOBEC3C using anti-APOBEC3C antibody (A07527-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOBEC3C antigen affinity purified polyclonal antibody (Catalog # A07527-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOBEC3C at approximately 23 kDa. The expected band size for APOBEC3C is at 23 kDa.
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