ProductsBack/Goat anti-CD11A / integrin alpha L

Goat anti-CD11A / integrin alpha L

EB09493

ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry

Product group Antibodies

TargetITGAL

Overview

Supplier
Everest Biotech
Product Name
Goat anti-CD11A / integrin alpha L Antibody
Delivery Days Customer
5
Applications
Western Blot, ELISA, ImmunoHistoChemistry
Applications Supplier
Pep-ELISA, WB, IHC
Certification
Research Use Only
Clonality
Polyclonal
Concentration
0.5 mg/ml
Gene ID3683
Target name
ITGAL
Target description
integrin subunit alpha L
Target synonyms
antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; CD11 antigen-like family member A; CD11A; integrin alpha-L; integrin gene promoter; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; LFA-1; LFA-1 alpha; LFA1A; LFA-1A; lymphocyte function-associated antigen 1, alpha polypeptide
Host
Goat
Scientific Description
RefSeq number(s): NP_002200.2; NP_001107852.1. Purification: Antigen affinity purified. Names and symbols: ITGAL; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); CD11A; LFA-1; LFA1A; LFA-1 alpha; antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; integrin alpha L; integrin g
Reactivity Supplier
Human
Storage Instruction
-20°C
UNSPSC
12352203

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Figure 1. Western blot analysis of CD11a using anti-CD11a antibody (A04466-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: JURKAT cell lysates, lane 2: CEM whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11a antigen affinity purified polyclonal antibody (Catalog # A04466-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11a at approximately 150KD. The expected band size for CD11a is at 129KD.

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$Paraformaldehyde-fixed, paraffin embedded rat adrendal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with \tRabbit Anti-Integrin alpha L/CD11a Polyclonal Antibody, Unconjugated (bs-1804R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.$