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ProductsBack/Goat anti-D-amino-acid oxidase (aa286-298) Antibody

Goat anti-D-amino-acid oxidase (aa286-298) Antibody

EB11100
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityBovine, Canine, Human, Porcine
TargetDAO
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-D-amino-acid oxidase (aa286-298) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID1610
  • Target name
    DAO
  • Target description
    D-amino acid oxidase
  • Target synonyms
    DAAO, DAMOX, OXDA, D-amino-acid oxidase
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001908.3. Purification: Antigen affinity purified. Names and symbols: DAO; D-amino-acid oxidase; DAAO; DAMOX; MGC35381; OXDA
  • Reactivity
    Bovine, Canine, Human, Porcine
  • Reactivity Supplier
    Human, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Nuzzo T, Punzo D, Devoto P, et al. The levels of the NMDA receptor co-agonist D-serine are reduced in the substantia nigra of MPTP-lesioned macaques and in the cerebrospinal fluid of Parkinson's disease patients. Sci Rep. 2019,9(1):8898. doi: 10.1038/s41598-019-45419-1
    Read this paper
  • Sasabe J, Miyoshi Y, Rakoff-Nahoum S, et al. Interplay between microbial d-amino acids and host d-amino acid oxidase modifies murine mucosal defence and gut microbiota. Nat Microbiol. 2016,1(10):16125. doi: 10.1038/nmicrobiol.2016.125
    Read this paper

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Figure 1. Western blot analysis of DAO using anti-DAO antibody (A00773-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAO antigen affinity purified polyclonal antibody (Catalog # A00773-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAO at approximately 39 kDa. The expected band size for DAO is at 39 kDa.
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