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ProductsBack/Goat anti-DAX1 / NR0B1

Goat anti-DAX1 / NR0B1

EB07778
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetNR0B1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-DAX1 / NR0B1 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID190
  • Target name
    NR0B1
  • Target description
    nuclear receptor subfamily 0 group B member 1
  • Target synonyms
    AHC; AHCH; AHX; DAX1; DAX-1; DSS; DSS-AHC critical region on the X chromosome protein 1; GTD; HHG; NROB1; nuclear hormone receptor; nuclear receptor DAX-1; nuclear receptor subfamily 0 group B member 1; SRXY2; truncated nuclear receptor subfamily 0 group B member 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000466.2. Purification: Antigen affinity purified. Names and symbols: NR0B1; DAX1; nuclear receptor subfamily 0, group B, member 1; AHC; AHCH; AHX; DAX-1; DSS; GTD; HHG; NROB1 ; dosage-sensitive sex reversal; gonadotropin deficiency; nuclear hormone receptor
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DAX-1/NR0B1 using anti-DAX-1/NR0B1 antibody (A01521-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAX-1/NR0B1 antigen affinity purified polyclonal antibody (Catalog # A01521-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAX-1/NR0B1 at approximately 48 kDa. The expected band size for DAX-1/NR0B1 is at 48 kDa.
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