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Western Blot Positive WB detected in: Hela whole cell lysate All lanes: NR0B1 antibody at 2.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 44 kDa Observed band size: 52 kDa
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: NR0B1 antibody at 2.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 44 kDa Observed band size: 52 kDa
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: NR0B1 antibody at 2.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 44 kDa Observed band size: 52 kDa

NR0B1 Antibody

CSB-PA016041LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNR0B1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NR0B1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID190
  • Target name
    NR0B1
  • Target description
    nuclear receptor subfamily 0 group B member 1
  • Target synonyms
    AHC; AHCH; AHX; DAX1; DAX-1; DSS; DSS-AHC critical region on the X chromosome protein 1; GTD; HHG; NROB1; nuclear hormone receptor; nuclear receptor DAX-1; nuclear receptor subfamily 0 group B member 1; SRXY2; truncated nuclear receptor subfamily 0 group B member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51843
  • Protein Name
    Nuclear receptor subfamily 0 group B member 1
  • Scientific Description
    Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DAX-1/NR0B1 using anti-DAX-1/NR0B1 antibody (A01521-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAX-1/NR0B1 antigen affinity purified polyclonal antibody (Catalog # A01521-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAX-1/NR0B1 at approximately 48 kDa. The expected band size for DAX-1/NR0B1 is at 48 kDa.
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