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Goat anti-DYRK1A

EB11483
Everest Biotech
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetDYRK1A
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-DYRK1A Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunoprecipitation: This product has been successfully used in IP on Human: Widowati et al, BMC Res Notes. 2018 May 15;11(1):297. PMID: 29764512.
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID1859
  • Target name
    DYRK1A
  • Target description
    dual specificity tyrosine phosphorylation regulated kinase 1A
  • Target synonyms
    dual specificity tyrosine-(Y)-phosphorylation regulated kinase 1A; dual specificity tyrosine-phosphorylation-regulated kinase 1A; dual specificity YAK1-related kinase; DYRK; DYRK1; HP86; MNB; mnb protein kinase homolog hp86; MNB/DYRK protein kinase; MNBH; MRD7; protein kinase minibrain homolog; serine/threonine kinase MNB; serine/threonine-specific protein kinase
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001387.2; NP_569120.1; NP_567824.1; NP_569122.1. GeneIDs all Nonhuman: 13548 (mouse); 25255 (rat);. Purification: Antigen affinity purified. Names and symbols: DYRK1A; dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A; DYRK; DYRK1; HP86; MNB; MNBH; MRD7; MNB/DYRK protein kinase; OTTHUMP00000109090; OTTHUMP00000109091; OTTHUMP00000109094; OTTHUMP00000174799; dual specificity YAK1-related kinase; d
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Mutational analysis of two residues in the DYRK homology box of the protein kinase DYRK1A. Widowati EW et al., 2018 May 15, BMC Res Notes
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Figure 1. Western blot analysis of DYRK1A using anti-DYRK1A antibody (A00878-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DYRK1A antigen affinity purified polyclonal antibody (Catalog # A00878-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DYRK1A at approximately 100 kDa. The expected band size for DYRK1A is at 86 kDa.
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