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ProductsBack/Goat anti-E2-230K / UBE2O

Goat anti-E2-230K / UBE2O

EB05761
Everest Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetUBE2O
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-E2-230K / UBE2O Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID63893
  • Target name
    UBE2O
  • Target description
    ubiquitin conjugating enzyme E2 O
  • Target synonyms
    E2-230K, (E3-independent) E2 ubiquitin-conjugating enzyme, E2/E3 hybrid ubiquitin-protein ligase UBE2O, ubiquitin carrier protein O, ubiquitin conjugating enzyme E2O, ubiquitin-conjugating enzyme E2 of 230 kDa, ubiquitin-conjugating enzyme E2-230K, ubiquitin-protein ligase O
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_071349.3. GeneIDs all Nonhuman: 217342 (mouse);. Purification: Antigen affinity purified. Names and symbols: UBE2O; ubiquitin-conjugating enzyme E2O; E2-230K; FLJ12878; KIAA1734
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UBE2O using anti-UBE2O antibody (A09217-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2O antigen affinity purified polyclonal antibody (Catalog # A09217-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBE2O at approximately 200 kDa. The expected band size for UBE2O is at 141 kDa.
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