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ProductsBack/UBE2O antibody [C1C2], Internal
UBE2O antibody [C1C2], Internal detects UBE2O protein at cytosol on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. UBE2O antibody [C1C2], Internal (GTX119315) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
UBE2O antibody [C1C2], Internal detects UBE2O protein at cytosol on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. UBE2O antibody [C1C2], Internal (GTX119315) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
UBE2O antibody [C1C2], Internal detects UBE2O protein at cytosol on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. UBE2O antibody [C1C2], Internal (GTX119315) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

UBE2O antibody [C1C2], Internal

GTX119315
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetUBE2O
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Overview

  • Supplier
    GeneTex
  • Product Name
    UBE2O antibody [C1C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID63893
  • Target name
    UBE2O
  • Target description
    ubiquitin conjugating enzyme E2 O
  • Target synonyms
    E2-230K, (E3-independent) E2 ubiquitin-conjugating enzyme, E2/E3 hybrid ubiquitin-protein ligase UBE2O, ubiquitin carrier protein O, ubiquitin conjugating enzyme E2O, ubiquitin-conjugating enzyme E2 of 230 kDa, ubiquitin-conjugating enzyme E2-230K, ubiquitin-protein ligase O
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9C0C9
  • Protein Name
    (E3-independent) E2 ubiquitin-conjugating enzyme
  • Scientific Description
    Catalyzes the covalent attachment of ubiquitin to other proteins.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Huang Y, Yang X, Lu Y, et al. UBE2O targets Mxi1 for ubiquitination and degradation to promote lung cancer progression and radioresistance. Cell Death Differ. 2021,28(2):671-684. doi: 10.1038/s41418-020-00616-8
    Read this paper

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Figure 1. Western blot analysis of UBE2O using anti-UBE2O antibody (A09217-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2O antigen affinity purified polyclonal antibody (Catalog # A09217-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBE2O at approximately 200 kDa. The expected band size for UBE2O is at 141 kDa.
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