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Goat anti-EHD1

EB06492
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetEHD1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-EHD1 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IF, IHC, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID10938
  • Target name
    EHD1
  • Target description
    EH domain containing 1
  • Target synonyms
    EH domain-containing protein 1; H-PAST; HPAST1; PAST; PAST homolog 1; PAST1; testilin
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001269373.1; NP_001269374.1. Purification: Antigen affinity purified. Names and symbols: EHD1; PAST; PAST1; H-PAST; HPAST1; EH-domain containing 1; testilin; FLJ42622; FLJ44618; OTTHUMP00000069747
  • Reactivity Supplier
    Human, Mouse, Rat, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Flow Cytometry analysis of U20S cells using anti-EHD1 antibody (A02168-1). Overlay histogram showing U20S cells stained with A02168-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EHD1 Antibody (A02168-1,1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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