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ProductsBack/Goat anti-ETF-4 / TEAD2

Goat anti-ETF-4 / TEAD2

EB09259
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetTEAD2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ETF-4 / TEAD2 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID8463
  • Target name
    TEAD2
  • Target description
    TEA domain transcription factor 2
  • Target synonyms
    ETF; TEA domain family member 2; TEAD-2; TEF4; TEF-4; transcriptional enhancer factor TEF-4
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_003589.1. GeneIDs all Nonhuman: 21677 (mouse); 294299 (rat);. Purification: Antigen affinity purified. Names and symbols: TEAD2; TEA domain family member 2; ETF; TEF-4; TEF4
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ETF/TEAD2 using anti-ETF/TEAD2 antibody (A09168-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETF/TEAD2 antigen affinity purified polyclonal antibody (Catalog # A09168-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ETF/TEAD2 at approximately 49KD. The expected band size for ETF/TEAD2 is at 49KD.
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