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WB analysis of mouse brain cell lysate using GTX34260 TEF4 antibody.
WB analysis of mouse brain cell lysate using GTX34260 TEF4 antibody.
WB analysis of mouse brain cell lysate using GTX34260 TEF4 antibody.

TEF4 antibody

GTX34260
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetTEAD2
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Overview

  • Supplier
    GeneTex
  • Product Name
    TEF4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:100-1:300. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8463
  • Target name
    TEAD2
  • Target description
    TEA domain transcription factor 2
  • Target synonyms
    ETF, TEAD-2, TEF-4, TEF4, transcriptional enhancer factor TEF-4, TEA domain family member 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15562
  • Protein Name
    Transcriptional enhancer factor TEF-4
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ETF/TEAD2 using anti-ETF/TEAD2 antibody (A09168-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETF/TEAD2 antigen affinity purified polyclonal antibody (Catalog # A09168-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ETF/TEAD2 at approximately 49KD. The expected band size for ETF/TEAD2 is at 49KD.
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