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IHC-P analysis of human breast cancer tissue using GTX34260 TEF4 antibody. Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Tris-EDTA, pH8.0 under high-pressure and temperature Dilution : 1:100
IHC-P analysis of human breast cancer tissue using GTX34260 TEF4 antibody. Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Tris-EDTA, pH8.0 under high-pressure and temperature Dilution : 1:100
IHC-P analysis of human breast cancer tissue using GTX34260 TEF4 antibody. Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Tris-EDTA, pH8.0 under high-pressure and temperature Dilution : 1:100

TEF4 antibody

GTX34260
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTEAD2
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Overview

  • Supplier
    GeneTex
  • Product Name
    TEF4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:100-1:300. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8463
  • Target name
    TEAD2
  • Target description
    TEA domain transcription factor 2
  • Target synonyms
    ETF, TEAD-2, TEF-4, TEF4, transcriptional enhancer factor TEF-4, TEA domain family member 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15562
  • Protein Name
    Transcriptional enhancer factor TEF-4
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ETF/TEAD2 using anti-ETF/TEAD2 antibody (A09168-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETF/TEAD2 antigen affinity purified polyclonal antibody (Catalog # A09168-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ETF/TEAD2 at approximately 49KD. The expected band size for ETF/TEAD2 is at 49KD.
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