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Goat anti-ETFA (aa139-152)

EB10173
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetETFA
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ETFA (aa139-152) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID2108
  • Target name
    ETFA
  • Target description
    electron transfer flavoprotein subunit alpha
  • Target synonyms
    EMA, GA2, MADD, electron transfer flavoprotein subunit alpha, mitochondrial, alpha-ETF, electron transfer flavoprotein alpha subunit, electron transfer flavoprotein, alpha polypeptide, epididymis secretory sperm binding protein, glutaric aciduria II, multiple acyl-CoA dehydrogenase deficiency
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000117.1; NP_001121188.1. GeneIDs all Nonhuman: 110842 (mouse); 300726 (rat);. Purification: Antigen affinity purified. Names and symbols: ETFA; electron-transfer-flavoprotein, alpha polypeptide; EMA; GA2; MADD; electron transfer flavoprotein alpha-subunit; electron transfer flavoprotein, alpha polypeptide; glutaric aciduria II
  • Reactivity Supplier
    Human, Mouse, Rat, Pig, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Tomilov A, Tomilova N, Shan Y, et al. p46Shc Inhibits Thiolase and Lipid Oxidation in Mitochondria. J Biol Chem. 2016,291(24):12575-12585. doi: 10.1074/jbc.M115.695577
    Read this paper

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Figure 1. Western blot analysis of ETFA using anti-ETFA antibody (A05572-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human U937 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETFA antigen affinity purified polyclonal antibody (Catalog # A05572-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ETFA at approximately 35 kDa. The expected band size for ETFA is at 35 kDa.
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