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ProductsBack/Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) Polyclonal Antibody

Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) Polyclonal Antibody

CAU22824
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetETFA
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Overview

  • Supplier
    Biomatik
  • Product Name
    Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2108
  • Target name
    ETFA
  • Target description
    electron transfer flavoprotein subunit alpha
  • Target synonyms
    EMA, GA2, MADD, electron transfer flavoprotein subunit alpha, mitochondrial, alpha-ETF, electron transfer flavoprotein alpha subunit, electron transfer flavoprotein, alpha polypeptide, epididymis secretory sperm binding protein, glutaric aciduria II, multiple acyl-CoA dehydrogenase deficiency
  • Host
    Rabbit
  • Protein IDP13804
  • Protein Name
    Electron transfer flavoprotein subunit alpha, mitochondrial
  • Scientific Description
    The Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: ETFA antibody at 4.34microg/ml Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lane 4: A549 whole cell lysate Lane 5: HepG2 whole cell lysate Lane 6: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 36, 31 kDa Observed band size: 36 kDa
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Figure 1. Western blot analysis of ETFA using anti-ETFA antibody (A05572-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human U937 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETFA antigen affinity purified polyclonal antibody (Catalog # A05572-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ETFA at approximately 35 kDa. The expected band size for ETFA is at 35 kDa.
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