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ProductsBack/Goat anti-HADH / HADHSC

Goat anti-HADH / HADHSC

EB08200
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetHADH
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-HADH / HADHSC Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID3033
  • Target name
    HADH
  • Target description
    hydroxyacyl-CoA dehydrogenase
  • Target synonyms
    HAD, HADH1, HADHSC, HCDH, HHF4, MSCHAD, SCHAD, hydroxyacyl-coenzyme A dehydrogenase, mitochondrial, L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain, medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase, short-chain 3-hydroxyacyl-CoA dehydrogenase, testis secretory sperm-binding protein Li 203a
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_005318.2. GeneIDs all Nonhuman: 15107 (mouse);. Purification: Antigen affinity purified. Names and symbols: HADH; HADHSC; hydroxyacyl-Coenzyme A dehydrogenase; HAD; HADH1; HHF4; M/SCHAD; MGC8392; SCHAD; L-3-hydroxyacyl-Coenzyme A dehydrogenase; L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HADH using anti-HADH antibody (A03650-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HADH antigen affinity purified polyclonal antibody (Catalog # A03650-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HADH at approximately 34 kDa. The expected band size for HADH is at 34 kDa.
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