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Western blot All lanes: HADH antibody at 2.38microg/ml Lane 1: Mouse heart tissue Lane 2: Mouse liver tissue Lane 3: Mouse kidney tissue Lane 4: Mouse skeletal muscle tissue Lane 5: NIH/3T3 whole cell lysate Lane 6: Hela whole cell lysate Lane 7: 293T whole cell lysate Lane 8: A431 whole cell lysate Lane 9: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 35, 43, 37 kDa Observed band size: 35 kDa
Western blot All lanes: HADH antibody at 2.38microg/ml Lane 1: Mouse heart tissue Lane 2: Mouse liver tissue Lane 3: Mouse kidney tissue Lane 4: Mouse skeletal muscle tissue Lane 5: NIH/3T3 whole cell lysate Lane 6: Hela whole cell lysate Lane 7: 293T whole cell lysate Lane 8: A431 whole cell lysate Lane 9: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 35, 43, 37 kDa Observed band size: 35 kDa
Western blot All lanes: HADH antibody at 2.38microg/ml Lane 1: Mouse heart tissue Lane 2: Mouse liver tissue Lane 3: Mouse kidney tissue Lane 4: Mouse skeletal muscle tissue Lane 5: NIH/3T3 whole cell lysate Lane 6: Hela whole cell lysate Lane 7: 293T whole cell lysate Lane 8: A431 whole cell lysate Lane 9: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 35, 43, 37 kDa Observed band size: 35 kDa

HADH Antibody

CSB-PA614992ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetHADH
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Overview

  • Supplier
    Cusabio
  • Product Name
    HADH Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3033
  • Target name
    HADH
  • Target description
    hydroxyacyl-CoA dehydrogenase
  • Target synonyms
    HAD, HADH1, HADHSC, HCDH, HHF4, MSCHAD, SCHAD, hydroxyacyl-coenzyme A dehydrogenase, mitochondrial, L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain, medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase, short-chain 3-hydroxyacyl-CoA dehydrogenase, testis secretory sperm-binding protein Li 203a
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16836
  • Protein Name
    Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial
  • Scientific Description
    Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HADH using anti-HADH antibody (A03650-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HADH antigen affinity purified polyclonal antibody (Catalog # A03650-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HADH at approximately 34 kDa. The expected band size for HADH is at 34 kDa.
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