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ProductsBack/Goat anti-HAO2 (aa54-67) Antibody

Goat anti-HAO2 (aa54-67) Antibody

EB12813
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetHAO2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-HAO2 (aa54-67) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID51179
  • Target name
    HAO2
  • Target description
    hydroxyacid oxidase 2
  • Target synonyms
    GIG16, HAOX2, 2-Hydroxyacid oxidase 2, (S)-2-hydroxy-acid oxidase, peroxisomal, cell growth-inhibiting gene 16 protein, glycolate oxidase, hydroxyacid oxidase 2 (long chain), long chain alpha-hydroxy acid oxidase, long-chain L-2-hydroxy acid oxidase
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_057611.1. Purification: Antigen affinity purified. Names and symbols: HAO2; hydroxyacid oxidase 2 (long chain); GIG16; HAOX2; (S)-2-hydroxy-acid oxidase, peroxisomal; cell growth-inhibiting gene 16 protein; glycolate oxidase; hydroxyacid oxidase 2; long chain alpha-hydroxy acid oxidase; long-chain L-2-hydroxy acid oxidase
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of HAO2 using anti-HAO2 antibody (A11930-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAO2 antigen affinity purified polyclonal antibody (Catalog # A11930-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HAO2 at approximately 39 kDa. The expected band size for HAO2 is at 39 kDa.
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