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Western Blot Positive WB detected in: MCF-7 whole cell lysate, Rat kidney tissue, Mouse kidney tissue All lanes: HAO2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39, 41 kDa Observed band size: 39 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate, Rat kidney tissue, Mouse kidney tissue All lanes: HAO2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39, 41 kDa Observed band size: 39 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate, Rat kidney tissue, Mouse kidney tissue All lanes: HAO2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39, 41 kDa Observed band size: 39 kDa

HAO2 Antibody

CSB-PA882145LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHAO2
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Overview

  • Supplier
    Cusabio
  • Product Name
    HAO2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51179
  • Target name
    HAO2
  • Target description
    hydroxyacid oxidase 2
  • Target synonyms
    GIG16, HAOX2, 2-Hydroxyacid oxidase 2, (S)-2-hydroxy-acid oxidase, peroxisomal, cell growth-inhibiting gene 16 protein, glycolate oxidase, hydroxyacid oxidase 2 (long chain), long chain alpha-hydroxy acid oxidase, long-chain L-2-hydroxy acid oxidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NYQ3
  • Protein Name
    2-Hydroxyacid oxidase 2
  • Scientific Description
    Catalyzes the oxidation of L-alpha-hydroxy acids as well as, more slowly, that of L-alpha-amino acids.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HAO2 using anti-HAO2 antibody (A11930-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAO2 antigen affinity purified polyclonal antibody (Catalog # A11930-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HAO2 at approximately 39 kDa. The expected band size for HAO2 is at 39 kDa.
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