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Anti-PC1 / ENPP1 (C-term) Antibody

119-12896
RayBiotech
ApplicationsELISA
Product group Antibodies
TargetENPP1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Goat Anti-Human PC1 / ENPP1 (C-Terminus)
  • Delivery Days Customer
    4
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5167
  • Target name
    ENPP1
  • Target description
    ectonucleotide pyrophosphatase/phosphodiesterase 1
  • Target synonyms
    ARHR2, COLED, M6S1, NPP1, NPPS, PC-1, PCA1, PDNP1, ectonucleotide pyrophosphatase/phosphodiesterase family member 1, E-NPP 1, Ly-41 antigen, NPPase, alkaline phosphodiesterase 1, alkaline phosphodiesterase I, membrane component, chromosome 6, surface marker 1, nucleotide diphosphatase, nucleotide pyrophosphatase, phosphodiesterase I/nucleotide pyrophosphatase 1, plasma-cell membrane glycoprotein 1, plasma-cell membrane glycoprotein PC-1
  • Host
    Goat
  • Protein IDP22413
  • Protein Name
    Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
  • Scientific Description
    PC1 / ENPP1 Goat anti-Human Polyclonal (C-Terminus) (Unconjugated) Antibody, (50 ug)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ENPP1/PC1 using anti-ENPP1/PC1 antibody (A01342-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENPP1/PC1 antigen affinity purified polyclonal antibody (Catalog # A01342-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENPP1/PC1 at approximately 130-150 kDa. The expected band size for ENPP1/PC1 is at 105 kDa.
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Lane 1: Human HepG2 cell lysates; Lane 2: Human Huvec cell lysates probed with ENPP1 Polyclonal Antibody, Unconjugated (bs-1760R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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