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Goat anti-IFT88 / Polaris

EB07088
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetIFT88
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-IFT88 / Polaris Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the nucleus and less staining in the cytoplasm of HepG2 cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID8100
  • Target name
    IFT88
  • Target description
    intraflagellar transport 88
  • Target synonyms
    D13S1056E; DAF19; hTg737; intraflagellar transport 88 homolog; intraflagellar transport protein 88 homolog; polaris homolog; probe hTg737 (polycystic kidney disease, autosomal recessive); recessive polycystic kidney disease protein Tg737 homolog; testicular tissue protein Li 93; tetratricopeptide repeat domain 10; tetratricopeptide repeat protein 10; TG737; TPR repeat protein 10; TTC10
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_783195.2; NP_006522.2, NP_001340507.1; NP_001340498.1. GeneIDs all Nonhuman: 21821 (mouse); 305918 (rat);. Purification: Antigen affinity purified. Names and symbols: IFT88; polaris; TTC10; tetratricopeptide repeat domain 10; HGNC:20606; D13S1056E; DAF19; IFT88; MGC26259; RP11-172H24.2; TG737; hTg737; TPR repeat protein 10; Probe hTg737 (polycystic kidney disease, autosomal recessive); Tg737 protein; intraflagellar tra
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice. Miyoshi K et al., 2009 Oct, FASEB J
    Read more

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Figure 1. Western blot analysis of IFT88 using anti-IFT88 antibody (A06814-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT88 antigen affinity purified polyclonal antibody (Catalog # A06814-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFT88 at approximately 94KD. The expected band size for IFT88 is at 94KD.
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