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ProductsBack/TG737 / IFT88 Antibody

TG737 / IFT88 Antibody

LS-C748117
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetIFT88
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TG737 / IFT88 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 92kDa/93kDa/94kDa, while the observed MW by Western blot was 108kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 92kDa/93kDa/94kDa, while the observed MW by Western blot was 108kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8100
  • Target name
    IFT88
  • Target description
    intraflagellar transport 88
  • Target synonyms
    D13S1056E, DAF19, TG737, TTC10, hTg737, intraflagellar transport protein 88 homolog, TPR repeat protein 10, intraflagellar transport 88 homolog, polaris homolog, probe hTg737 (polycystic kidney disease, autosomal recessive), recessive polycystic kidney disease protein Tg737 homolog, testicular tissue protein Li 93, tetratricopeptide repeat domain 10, tetratricopeptide repeat protein 10
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IFT88 using anti-IFT88 antibody (A06814-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFT88 antigen affinity purified polyclonal antibody (Catalog # A06814-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFT88 at approximately 94KD. The expected band size for IFT88 is at 94KD.
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