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ProductsBack/Goat anti-Karyopherin (importin) beta 1

Goat anti-Karyopherin (importin) beta 1

EB07018
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetKPNB1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Karyopherin (importin) beta 1 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID3837
  • Target name
    KPNB1
  • Target description
    karyopherin subunit beta 1
  • Target synonyms
    IMB1; Impnb; importin 1; importin 90; importin beta-1 subunit; importin subunit beta-1; IPO1; IPOB; karyopherin (importin) beta 1; NTF97; nuclear factor p97; pore targeting complex 97 kDa subunit; PTAC97
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002256.2; NP_001263382.1. GeneIDs all Nonhuman: 16211 (mouse); 24917 (rat);. Purification: Antigen affinity purified. Names and symbols: KPNB1; karyopherin (importin) beta 1; HGNC:6400; IMB1; IPOB; Impnb; MGC2155; MGC2156; MGC2157; NTF97; importin 90; importin beta-1 subunit; karyopherin beta 1; nuclear factor p97; KapB1; IPO1
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KPNB1 using anti-KPNB1 antibody (A01851-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KPNB1 antigen affinity purified polyclonal antibody (Catalog # A01851-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KPNB1 at approximately 97 kDa. The expected band size for KPNB1 is at 97 kDa.
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