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The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA244093(KPNB1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA244093(KPNB1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA244093(KPNB1 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)

KPNB1 Antibody

CSB-PA244093
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetKPNB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    KPNB1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3837
  • Target name
    KPNB1
  • Target description
    karyopherin subunit beta 1
  • Target synonyms
    IMB1; Impnb; importin 1; importin 90; importin beta-1 subunit; importin subunit beta-1; IPO1; IPOB; karyopherin (importin) beta 1; NTF97; nuclear factor p97; pore targeting complex 97 kDa subunit; PTAC97
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14974
  • Protein Name
    Importin subunit beta-1
  • Scientific Description
    Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KPNB1 using anti-KPNB1 antibody (A01851-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KPNB1 antigen affinity purified polyclonal antibody (Catalog # A01851-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KPNB1 at approximately 97 kDa. The expected band size for KPNB1 is at 97 kDa.
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