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ProductsBack/Goat anti-LCK (aa39-52)

Goat anti-LCK (aa39-52)

EB11387
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetLCK
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-LCK (aa39-52) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID3932
  • Target name
    LCK
  • Target description
    LCK proto-oncogene, Src family tyrosine kinase
  • Target synonyms
    IMD22; leukocyte C-terminal Src kinase; LSK; lymphocyte cell-specific protein-tyrosine kinase; p56(LSTRA) protein-tyrosine kinase; p56lck; pp58lck; proto-oncogene tyrosine-protein kinase LCK; t cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyrosine-protein kinase Lck; YT16
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_005347.3. GeneIDs all Nonhuman: 16818 (mouse); 313050 (rat);. Purification: Antigen affinity purified. Names and symbols: LCK; lymphocyte-specific protein tyrosine kinase; LSK; YT16; p56lck; pp58lck; OTTHUMP00000008640; OTTHUMP00000008740; OTTHUMP00000008741; T-lymphocyte specific protein tyrosine kinase p56lck; leukocyte C-terminal Src kinase; lymphocyte cell-specific prote
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Lck using anti-Lck antibody (PB9808). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lck antigen affinity purified polyclonal antibody (Catalog # PB9808) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lck at approximately 58 kDa. The expected band size for Lck is at 58 kDa.
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