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Lck Antibody (YA712, Mouse)

HY-P80743
MedChem Express
TargetLCK
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Lck Antibody (YA712, Mouse)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3932
  • Target name
    LCK
  • Target description
    LCK proto-oncogene, Src family tyrosine kinase
  • Target synonyms
    IMD22; leukocyte C-terminal Src kinase; LSK; lymphocyte cell-specific protein-tyrosine kinase; p56(LSTRA) protein-tyrosine kinase; p56lck; pp58lck; proto-oncogene tyrosine-protein kinase LCK; t cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyrosine-protein kinase Lck; YT16
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Lck Antibody (YA712) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Lck.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Lck using anti-Lck antibody (PB9808). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lck antigen affinity purified polyclonal antibody (Catalog # PB9808) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lck at approximately 58 kDa. The expected band size for Lck is at 58 kDa.
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