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ProductsBack/Goat anti-LIS1 / PAFAH1B1

Goat anti-LIS1 / PAFAH1B1

EB05439
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetPAFAH1B1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-LIS1 / PAFAH1B1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5048
  • Target name
    PAFAH1B1
  • Target description
    platelet activating factor acetylhydrolase 1b regulatory subunit 1
  • Target synonyms
    LIS1; LIS2; lissencephaly 1 protein; MDCR; MDS; NudF; PAFAH; platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa); platelet-activating factor acetylhydrolase IB subunit beta
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000421.1. Purification: Antigen affinity purified. Names and symbols: PAFAH1B1; LIS1; platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa; MDCR; PAFAH; lissencephaly 1 protein; Platelet-activating factor acetylhydrolase, isoform 1B, alpha subunit; platelet-activating factor acetylhydrolase, isoform I
  • Reactivity Supplier
    Human, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LIS1/PAFAH1B1 using anti-LIS1/PAFAH1B1 antibody (A01273-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIS1/PAFAH1B1 antigen affinity purified polyclonal antibody (Catalog # A01273-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIS1/PAFAH1B1 at approximately 47 kDa. The expected band size for LIS1/PAFAH1B1 is at 47 kDa.
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