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ProductsBack/PAFAH1B1 / LIS1 Antibody

PAFAH1B1 / LIS1 Antibody

LS-C747734
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetPAFAH1B1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PAFAH1B1 / LIS1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 16kDa/46kDa, while the observed MW by Western blot was 47kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 16kDa/46kDa, while the observed MW by Western blot was 47kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5048
  • Target name
    PAFAH1B1
  • Target description
    platelet activating factor acetylhydrolase 1b regulatory subunit 1
  • Target synonyms
    LIS1, LIS2, MDCR, MDS, NudF, PAFAH, platelet-activating factor acetylhydrolase IB subunit beta, lissencephaly 1 protein, platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of LIS1/PAFAH1B1 using anti-LIS1/PAFAH1B1 antibody (A01273-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIS1/PAFAH1B1 antigen affinity purified polyclonal antibody (Catalog # A01273-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIS1/PAFAH1B1 at approximately 47 kDa. The expected band size for LIS1/PAFAH1B1 is at 47 kDa.
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