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ProductsBack/Goat anti-M1 mAChR / CHRM1

Goat anti-M1 mAChR / CHRM1

EB07398
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCHRM1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-M1 mAChR / CHRM1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID1128
  • Target name
    CHRM1
  • Target description
    cholinergic receptor muscarinic 1
  • Target synonyms
    HM1, M1, M1R, muscarinic acetylcholine receptor M1, acetylcholine receptor, muscarinic 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000729.2. GeneIDs all Nonhuman: 12669 (mouse); 25229 (rat);. Purification: Antigen affinity purified. Names and symbols: CHRM1; M1 mAChR; cholinergic receptor, muscarinic 1 ; HM1; M1; MGC30125 ; muscarinic acetylcholine receptor M1
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CHRM1 using anti-CHRM1 antibody (A04081). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: mouse Neuro-2A whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHRM1 antigen affinity purified polyclonal antibody (Catalog # A04081) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHRM1 at approximately 55KD. The expected band size for CHRM1 is at 51KD.
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