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L1 rat kidney lysates, L2 rat brain lysates probed (bs-1150R) Anti-ChRM1/Acetylcholine receptor(M1) Polyclonal, Unconjugated at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 51kD.
L1 rat kidney lysates, L2 rat brain lysates probed (bs-1150R) Anti-ChRM1/Acetylcholine receptor(M1) Polyclonal, Unconjugated at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 51kD.
L1 rat kidney lysates, L2 rat brain lysates probed (bs-1150R) Anti-ChRM1/Acetylcholine receptor(M1) Polyclonal, Unconjugated at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 51kD.

muscarinic Acetylcholine Receptor 1 Antibody

BS-1150R
Bioss
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Equine, Human, Mouse, Porcine, Rat
TargetCHRM1
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Overview

  • Supplier
    Bioss
  • Product Name
    muscarinic Acetylcholine Receptor 1 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1128
  • Target name
    CHRM1
  • Target description
    cholinergic receptor muscarinic 1
  • Target synonyms
    HM1, M1, M1R, muscarinic acetylcholine receptor M1, acetylcholine receptor, muscarinic 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11229
  • Protein Name
    Muscarinic acetylcholine receptor M1
  • Reactivity
    Bovine, Equine, Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zhang W, Chen J, Zhao Y, et al. The inhibitory effect of melatonin on mammary function of lactating dairy goats†. Biol Reprod. 2019,100(2):455-467. doi: 10.1093/biolre/ioy223
    Read this paper

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Figure 1. Western blot analysis of CHRM1 using anti-CHRM1 antibody (A04081). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: mouse Neuro-2A whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHRM1 antigen affinity purified polyclonal antibody (Catalog # A04081) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHRM1 at approximately 55KD. The expected band size for CHRM1 is at 51KD.
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