Bio-Connect

Goat anti-NEFL (aa330-343)

EB13051
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetNEFL
100 ug
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-NEFL (aa330-343) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID4747
  • Target name
    NEFL
  • Target description
    neurofilament light chain
  • Target synonyms
    CMT1F; CMT2E; CMTDIG; light molecular weight neurofilament protein; neurofilament light polypeptide; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light polypeptide 68kDa; NF68; NFL; NF-L; PPP1R110; protein phosphatase 1, regulatory subunit 110
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_006149.2. GeneIDs all Nonhuman: 18039 (mouse); 83613 (rat);. Purification: Antigen affinity purified. Names and symbols: NEFL; neurofilament, light polypeptide; CMT1F; CMT2E; NF-L; NF68; NFL; PPP1R110; light molecular weight neurofilament protein; neurofilament protein, light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament, light polypeptid
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetNEFL
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
TargetNEFL
  • SizePrice
Product group Antibodies
Assay Genie
TargetNEFL
  • SizePrice
Western Blot analysis of Jurkat cells using NF-L Polyclonal Antibody
Product group Antibodies
Cusabio
NEFL AntibodyCSB-PA004743
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetNEFL
  • SizePrice
Product group Antibodies
Biosynth
  • SizePrice
Product group Antibodies
Absolute Antibody
ApplicationsELISA
TargetNEFL
  • SizePrice
Product group Antibodies
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetNEFL
  • SizePrice
Immunohistochemical staining of human cerebellum shows strong cytoplasmic immunoreactivity in cell bodies and proximal dendrites of Purkinje cells.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNEFL
  • SizePrice
Product group Antibodies
Nordic-MUbio
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetNEFL
  • SizePrice
Figure 1. Western blot analysis of NEFL/NF-L using anti-NEFL/NF-L antibody (A02482-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEFL/NF-L antigen affinity purified polyclonal antibody (Catalog # A02482-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEFL/NF-L at approximately 72KD. The expected band size for NEFL/NF-L is at 72KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetNEFL
  • SizePrice